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EFFECTS OF TWO TYPES OF RESTRAINT STRESS ON BEHAVIOR OF RATS IN THE Y-MAZE AND OPEN FIELD S. Hynie, D. Lojkov, M. Koupilov, P. Sda, V. Klenerov Charles University in Prague, First Medical Faculty, Institute of Pharmacology, Prague, Czech Republic Various stressors have been shown to exert modulatory effects on animal cognition. The exposure of rats to inescapable stress has been demonstrated to disrupt cognitive processes in several models of dissociative or spatial learning tasks. The aim of this study was to examine the differences in response to two types of restraint stress in two behavioral tests. Rats were exposed for 1 hour to two types of restraint stressors 1 ; , namely restraint stress alone MO ; or restraint combined with cold-water stress IMO + C ; . the first series of experiments, learned discriminatory avoidance response in Y-maze was used as a model of memory; in this device we tested the differences between Wistar and Lewis rats, the latter having decreased activity of HPA axis. After exposure of rats to stressors the latency to enter the safe arm was recorded in 10 daily trials. In the second series of experiments we used "open field" test as basic information on behavior of Wistar rats. Rats were gently places in the right rear corner of the box and allowed to explore the arena for 15 min. The following behavioral parameters were recorded: a ; crossing, b ; immobility, and c ; rearing. All data were analyzed by ANOVA and post-tests. In the Y-maze both stressors prolonged the avoidance latencies for 2 or 3 days in Wistar and Lewis rats, respectively. In Lewis but not in Wistar rats the latency increased more after IMO + C compared to IMO stress. In contrast to minor differences between the effects of IMO and IMO + C on the performance of rats in the Y-maze, the open field experiments showed strong suppression of the exploratory behavior after IMO + C and the weaker effect of IMO itself. The exposure to IMO + C, terminating 240 or 60 min before the open field testing, practically abolished all motor activities. In the second open field test, performed without application of stress 7 days later, rats displayed a comparable exploratory activity. In summary, our data indicate substantial differences in response of rats to two types of restraint stressors in two behavioral tests. These findings can be utilized in other behavioral tests following interactions of stress with effects of drugs on cognitive functions.
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